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Week 2 Lab Activity

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Q Week 2 Lab: Microscopy & Searching for Scientific Literature To craft your responses for the following questions, refer to the activities in the Week 2 Lab Instructions available on the course Canvas site. Part A: Basic Microscope Techniques Microscope Tutorial 1. Explain why it is important to use only the fine focus knob at high powers. 2. Why is it important to center your slide in the field of view when using the scanning objective?3. What is the iris diaphragm aperture? What does it do, and when should you adjust it?4. When would you use the oil immersion lens? What power/magnification is the oil immersion lens?5. What kind of specimen would you use a compound microscope to view versus the kind of specimen that you would use a stereo microscope to view?

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1.Response: At high powers, the objective remains usually very close to the specimen or stage. So, if we use only fine focus knob, nothing will damage. Instead, if we use coarse knob, it can break the slide and damage the objective. So, it is very important. 2.Response: It is important to ensure the increased magnification. As the field of view becomes very small and if we do not center the slide, the object may disappear from the field of view in higher magnification. By adjusting the side in the center, we can view the object best.